Module Delivery Methods
C-2. Nanoneedle-Based in-Planta Delivery of Genome-Editing Modules
Delivery of genome editing modules to individual plants using nanoneedles
Molecular delivery by mechanoporation
C. Nakamura, AIST
chikashi-nakamuraaist.go.jp
Technical Description
Summary
We develop a technique for genome editing by physical insertion and direct material delivery into plant tissues, including stem apical meristematic tissue, using nanoneedle arrays of ultra-fine needles of high aspect ratio.
Application Destination
- Any plant species for which the host vector system has not yet been developed.
- It can be manipulated against microtissue such as stem apical meristematic tissue.
Reference Material
- JP2020-053154 “Microneedle array and a method for introducing substances into plant cells using the array”
(filed March 24, 2020; unpublished)
Contact Information
Chikashi Nakamura, Cellular and Molecular Biotechnology Research Institute, AIST
- E-mail: chikashi-nakamuraaist.go.jp
- URL: https://www.aist.go.jp/