Genome Modification Technologies
B-5. RNA-Binding PPR Proteins for Organellar Genome Editing

Transcriptome engineering by designer PPR protein
In vivo control of translation and splicing

T. Nakamura, Kyushu Univ.
agr.kyushu-u.ac.jp

Technical Description

Summary:

We provide “designer RNA binding protein using PPR motifs” and the related application, including splicing and translation, to edit or engineering in vivo RNA, without DNA alternation.

Technical advantages:

  • PPR is a versatile RNA engineering technique (patent approved).
  • Change of genome function at RNA level with no alternation of genomic DNA
  • The technique enables spatiotemporal regulation of essential RNA in vivo
Example of application: Transcriptome engineering to obtain valuable metabolite in plant

Application Destination

  • Engineering of metabolic pathway for production of valuable materials
  • Pharmaceutical use targeting aberrant endogenous RNA
  • Diagnosis and therapeutic use against RNA virus

Reference Material

  • Designer RNA binding protein using PPR motifs (WO/2013/058404)
  • Translational control by designer PPR protein (2016-120524)

Contact Information

Takahiro NAKAMURA, Kyushu University