Genome Modification Technologies
B-4. Organellar Genome Editing Technology

Organellar genome editing technology
Organellar genome editing (gene knock-in) using organellar localizing fusion peptide

T. Yoshizumi, Takasaki Univ. Health & Welfare
takasaki-u.ac.jp

Technical Description

Plant cells have 2 organelles such as chloroplasts and mitochondria which show an advantage in bioproduction of industrial and medical substances. We are developing selective organellar genome editing technology using organellar localization fusion peptides. Using this technology, exogenous DNA is able to be delivered only into target organelles. Additionally this is the only technology of mitochondrial genome editing in plants.

Application Destination

  • Seed company
  • Agricultural biotechnology company

Reference Material

  • Yoshizumi et al., (2018) Selective Gene Delivery for Integrating Exogenous DNA into Plastid and Mitochondrial Genomes Using Peptide-DNA Complexes. Biomacromolecules 19: 1582-1591
  • Kimura et al., (2019) A centrifugation-assisted peptide-mediated gene transfer method for high-throughput analyses. Plant Biotechnol. 36: 49-52

Contact Information

Takasaki University of Health and Welfare