DNA Recognition Modules
A-4. Development of a New RNA-Based Genome-Editing Technology

Development of a Novel Genome-Editing Method from Japan
Confirmation of PODiR system in plants

H. Maseda, AIST Kansai
aist.go.jp

Technical Description

  1. PODiR system?
    The PODiR system is one of the bacterial antibiotic resistance acquisition systems, and its presence is expected not only in bacteria but also in animal cells and plant cells. By applying this system, genome editing different from CRISPR-Cas9 can be expected.
  2. PODiR system for plants (cells)
    Assay cells are created and implemented to confirm that the PODiR system works in microorganisms, animals (cells), plants (cells), etc.

Application Destination

  • General breeding of living cells and things
  • Implementation of precise gene therapy
  • Creation of highly functional cells for bioenergy production

Reference Material

  • Tokugan2015-111458 新規発現誘導システムを可能にする真核細胞用発現カセット
  • Tokugan2015-149826 タンパク質発現方法
  • Tokugan2018-533576 ゲノム編集方法

Contact Information

National Institute of Advanced Industrial Science and Technology