DNA Recognition Modules
A-1. DNA-Binding PPR Proteins to Promote Practical Application of Genome-Editing Techniques
Development of DNA binding module
Establishment of technology for genome editing using DNA-binding PPR proteins
Y. Yagi, EditForce, Inc.
yusu-yageditforce.jp
Technical Description
Summary:
We have original design method for sequence-specific DNA binding protein using PPR protein. This can provide a molecule that can be used to knock out any target gene (genome editing).
Technical advantages:
- DNA binding module that are completely different from Zinc Finger, TALE and CRISPR systems
- Unique domestic technology with basic patents.
PPR: Original Sequence-Specific DNA Binding Module
- 1 protein motif (35 amino acids) binds to 1 base
- Programable DNA sequence specificity
- DNase fused PPR proteins can apply to genome editing.
Application Destination
- Modification of secondary metabolite production pathways
- Creation of genome-edited organisms of genes that cannot be targeted by existing methods
Reference Material
- DNA BINDING PROTEIN USING PPR MOTIF, AND USE THEREOF (WO2014175284A1)
Contact Information
Yusuke YAGI, EditForce, Inc.
- E-mail: yusu-yageditforce.jp
- URL: https://www.editforce.co.jp